Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky).

Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus.

Fifteen new polymorphic microsatellite markers were developed for Culter alburnus. In 32 individuals representing a wild population of the Danjiangkou Reservoir, Hubei, China, the number of alleles at these loci varied between 2 and 10, with an average of 5.5. The average observed and expected heterozygosities were 0.664 and 0.681, respectively. The polymorphism information content of 11 loci w...

Dissecting the role of transforming growth factor-β1 in topmouth culter immunobiological activity: a fundamental functional analysis

Transforming growth factor-β1 (TGF-β1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-β1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-β1 molecule in the topmouth culter. TGF-β1 is highly expressed in immune-related tissues of the culter, including the thy...

Triploid Intersexuality in Drosophila Americana Spencer.

Microsatellite Isolation and Characterization in Pomegranate (Punica granatum L.)

Development of microsatellite markers has been an increasing trend in crop genetic studies because oftheir applicability in breeding programs. Here we report the development of inter simple sequencerepeat (SSRs) in pomegranate (Punica granatum L.) using an enrichment method that makes use of magneticbeads. Enriched genomic libraries with AG and ATG microsatellite motifs were c...

Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)

Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...